What is a "Premier Blocking Solution"? The Most Authoritative Explanation.
What is a blocking solution?
Blocking solutions can prevent background interference from different sources, including autofluorescence, endogenous enzyme activity, Fc receptors, or interactions of detection reagents with tissue/cell proteins and other macromolecules. Vector Laboratories offers blocking solutions for a variety of applications.How do I choose a blocking agent?
There are three factors to consider when choosing an appropriate blocking agent: antibody compatibility, compatibility of the protein of interest, and the detection system. Some common blocking buffers and tips for their use are listed below. Non-fat Milk powder (2.5-5% solution) is the most commonly used and cheapest blocking agent.What is the cheapest blocking agent?
Non-fat Milk powder (2.5-5% solution) is the most commonly used and cheapest blocking agent. Milk should not be used if the protein of interest is phosphorylated. Milk contains casein, a phosphoprotein that will bind to anti-phospho antibodies which causes non-specific binding and high background noise.What is a good blocking buffer?
Some common blocking buffers and tips for their use are listed below. Non-fat Milk powder (2.5-5% solution) is the most commonly used and cheapest blocking agent. Milk should not be used if the protein of interest is phosphorylated.
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